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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	hepatocellular cancer
ICD-0-3	NA
Methods	qRT-PCR, RNA pull-down, Colony formation assay, FCM assay, Dual luciferase reporter gene assay, in vivo experiments, Western blot assay
Sample	HCC tumor tissues, HCC cell lines HepG2, SMMC-7721, SK-HEP-1, and SNU-398, the normal liver cell line MIHA, and the human renal epithelial cell line HEK-293T
Expression Pattern	up-regulated
Function Description	In this study, we sought to predict the effects of a certain circular RNA (circRNA), hsa_circ_101280 (also known as hsa_circ_0100929 and hsa_circ_SLAIN1), on hepatocellular carcinoma (HCC) cells and to determine the potential mechanism. After screening differentially expressed circRNAs in HCC tissues through GEO data analysis , hsa_circ_101280 was found to be highly expressed, and its high expression was verified in HCC cell lines with qRT -PCR along with the low expression of its downstream miRNA miR -375. Colony formation and flow cytometry assays showed that both hsa_circ_101280 silencing and miR -375 overexpression restrained proliferation and promoted apoptosis in HCC cells. JAK2 was identified as a downstream mRNA target of mi R -375 by RNA pull -down and dual -luciferase reporter gene assays, its expression in HCC cell lines were positively regulated by hsa_circ_101280 and negatively by miR -375 expression. Furthermore, silencing of has_circ_101280 significantly inhibited the growth of HCC xenografts in nude mice, with the down -regulated expression of JAK2 .
Pubmed ID	30302825
Year	2018
Title	Hsa_circ_101280 promotes hepatocellular carcinoma by regulating miR-375/JAK2

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