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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	hepatocellular cancer
ICD-0-3	NA
Methods	RT-qPCR and RNase R treatment, Western blotting, Cell proliferation, migration and invasion assay, Immunohistochemistry and fluorescence in situ hybridization (FISH)
Sample	hepatocellular carcinoma tissue and paired adjacent non-tumorous tissues, The human normal liver L02 cell line and the HCC cell lines (Huh7, SMMC-7721, BEL-7402, HepG2 and Hep3B)
Expression Pattern	down-regulated
Function Description	we analyzed a cohort of 102 patients and found that expression of both ZKSCAN1 mRNA and circZKSCAN1 was significantly lower (P<0.05) in the HCC samples compared with that in matched adjacent non-tumorous tissues by reverse transcription PCR (RT-PCR). The low expression level of ZKSCAN1 was only associated with tumor size (P=0.032), while the cirZKSCAN1 levels varied in patients with different tumor numbers (P<0.01), cirhosis (P=0.031), vascular invasion (P=0.002) or MVI (P=0.002), as well as with the tumor grade (P<0.001). Silencing both ZKSCAN1 mRNA and circZKSCAN1 promoted cell proliferation, migration and invasion. In contrast, over-expression of both forms of RNAs repressed HCC progression in vivo and in vitro. Silencing or over expression of both forms of RNAs didn?? interfere with each other. RNA-seq revealed a very different molecular basis for the observed effects; ZKSCAN1 mRNA mainly regulated cellular metabolism, while circZKSCAN1 mediated several cancer-related signaling pathways, suggesting a nonredundant role for ZKSCAN1 mRNA and circRNA.
Pubmed ID	28211215
Year	2017
Title	ZKSCAN1 gene and its related circular RNA (circZKSCAN1) both inhibit hepatocellular carcinoma cell growth, migration and invasion but through different signaling pathways

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