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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug	MCF-7
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	breast cancer
ICD-0-3	C50
Methods	RNA-seq, qRT-PCR, Western blot, in vitro knockdown, RNAi
Sample	cell lines (MCF-7, ZR-75.1, T-47D)
Expression Pattern	up-regulated
Function Description	By the analysis of H3K27ac enrichment in hormone-deprived MCF-7 cells, we defined a set of Super Enhancers (SEs) occupied by apoERa, including one mapped in proximity of the DSCAM-AS1 lncRNA gene.This represents a paradigm of apoERa activity since its expression is largely unaffected by estrogenic treatment,despite the fact that E2 increases ERa binding on DSCAM-AS1 promoter. We validated the enrichment of apoERa, p300, GATA3, FoxM1 and CTCF at both DSCAM-AS1 TSS and at its associated SE by ChIP-qPCR. Furthermore, by analyzing MCF-7 ChIA-PET data and by 3C assays, we confirmed long range chromatin interaction between the SE and the DSCAM-AS1 TSS. Interestingly, CTCF and p300 binding showed an enrichment in hormone-depleted medium and in the presence of ERa, elucidating the dynamics of the estrogen-independent regulation of DSCAM-AS1 expression.The most significant down-regulated lncRNAs were RP11-68L18.1, MIR9-3HG, and LINC01016, whereas NKILA, AC144831.1, and LINC00657 were the most significant up-regulated lncRNAs
Pubmed ID	29462945
Year	2018
Title	Luminal lncRNAs Regulation by ERa-Controlled Enhancers in a Ligand-Independent Manner in Breast Cancer Cells.

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