Basic Information
| LncRNA/CircRNA Name | AFAP1-AS1 |
| Synonyms | AFAP1-AS, AFAP4AS |
| Region | GRCh38_4:7754090-7778928 |
| Ensemble | ENSG00000272620 |
| Refseq | NR_026892 |
Classification Information
| Regulatory Mechanism | Biological Function | Clinical Application | |||
|---|---|---|---|---|---|
| TF | Immune | Survival | |||
| Enhancer | Apoptosis | Drug | Cucurbitacin B | ||
| Variant | Cell Growth | Circulating | |||
| MiRNA | EMT | Metastasis | |||
| Methylation | Coding Ability | Recurrence |
Cancer&Entry Information
| Cancer Name | pancreatic cancer |
| ICD-0-3 | C25 |
| Methods | qPCR, Western blo, Luciferase reporter assay, other |
| Sample | PC tissues and cell lines (ASPC-1, BxPC-3, HPAC, MiaPaCa-2) |
| Expression Pattern | down-regulated |
| Function Description | Insights into the mechanisms of competing endogenous RNAs (ceRNAs) gained from bioinformatics analysis and luciferase activity assays showed that the epidermal growth factor receptor (EGFR) and AFAP1-AS1 directly compete for miR-146b-5p binding. CuB-induced high miR-146b-5p expression and inhibited the expression of AFAP1-AS1. In summary, reducing the expression of endogenous AFAP1-AS1 effectively increased the available concentration of miR-146b-5p in PC, whereas miR-146b-5p overexpression prevented the expression of endogenous AFAP1-AS1. In particular, we hypothesized that AFAP1-AS1 might act as a ceRNA, effectively becoming a sponge for miR-146b-5p, thereby activating the expression of the EGFR. Thus, CuB suppresses the proliferation, in vitro and in vivo, of PC cells through the ceRNA effect of AFAP1-AS1 on miR-146b-5p. |
| Pubmed ID | 30206930 |
| Year | 2019 |
| Title | Cucurbitacin B Suppresses Proliferation of Pancreatic Cancer Cells by ceRNA: Effect of miR-146b-5p and lncRNA-AFAP1-AS1 |
External Links
| Links for AFAP1-AS1 | GenBank HGNC NONCODE |
| Links for pancreatic cancer | OMIM COSMIC |