Basic Information
LncRNA/CircRNA Name | AFAP1-AS1 |
Synonyms | AFAP1-AS, AFAP4AS |
Region | GRCh38_4:7754090-7778928 |
Ensemble | ENSG00000272620 |
Refseq | NR_026892 |
Classification Information
Regulatory Mechanism | Biological Function | Clinical Application | |||
---|---|---|---|---|---|
TF | Immune | Survival | |||
Enhancer | Apoptosis | Drug | Cucurbitacin B | ||
Variant | Cell Growth | Circulating | |||
MiRNA | EMT | Metastasis | |||
Methylation | Coding Ability | Recurrence |
Cancer&Entry Information
Cancer Name | pancreatic cancer |
ICD-0-3 | C25 |
Methods | qPCR, Western blo, Luciferase reporter assay, other |
Sample | PC tissues and cell lines (ASPC-1, BxPC-3, HPAC, MiaPaCa-2) |
Expression Pattern | down-regulated |
Function Description | Insights into the mechanisms of competing endogenous RNAs (ceRNAs) gained from bioinformatics analysis and luciferase activity assays showed that the epidermal growth factor receptor (EGFR) and AFAP1-AS1 directly compete for miR-146b-5p binding. CuB-induced high miR-146b-5p expression and inhibited the expression of AFAP1-AS1. In summary, reducing the expression of endogenous AFAP1-AS1 effectively increased the available concentration of miR-146b-5p in PC, whereas miR-146b-5p overexpression prevented the expression of endogenous AFAP1-AS1. In particular, we hypothesized that AFAP1-AS1 might act as a ceRNA, effectively becoming a sponge for miR-146b-5p, thereby activating the expression of the EGFR. Thus, CuB suppresses the proliferation, in vitro and in vivo, of PC cells through the ceRNA effect of AFAP1-AS1 on miR-146b-5p. |
Pubmed ID | 30206930 |
Year | 2019 |
Title | Cucurbitacin B Suppresses Proliferation of Pancreatic Cancer Cells by ceRNA: Effect of miR-146b-5p and lncRNA-AFAP1-AS1 |
External Links
Links for AFAP1-AS1 | GenBank HGNC NONCODE |
Links for pancreatic cancer | OMIM COSMIC |