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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	oral squamous cell carcinoma
ICD-0-3	C06.9
Methods	qPCR, Luciferase reporter assay
Sample	human OSCC cell lines (Fadu, SCC-25, CAL-27, and Tca8113), one normal human oral cell line (Hs 680.Tg), OSCC tissues, para-carcinoma tissues
Expression Pattern	up-regulated
Function Description	The results of qRT-PCR proved that the expression level of FEZF1-AS1 in OSCC tissues was significantly higher than that of para-carcinoma tissues, and the difference was statistically significant. The pathological stage was significantly higher in patients with highexpression FEZF1-AS1 than those with low-expression FEZF1-AS1, while the overall survival rate was remarkably lower. The proliferation ability of cells in FEZF1-AS1 silencing group declined significantly when compared with the NC group. Similarly, qRT-PCR results verified that the expression of miR-196a in OSCC cell lines and tissues was significantly reduced as well. Meanwhile, the miR-196a expression was negatively correlated with FEZF1-AS1. Subsequent Luciferase reporter gene assay confirmed that overexpression of miR-196a could markedly reduce the activity of Luciferase containing wildtype FEZF1-AS1 vector rather than decrease the activity of Luciferase containing mutant-type vector or empty vector.
Pubmed ID	31378890
Year	2019
Title	Mechanism of lncRNA FEZF1-AS1 in promoting the occurrence and development of oral squamous cell carcinoma through targeting miR-196a

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