Search Result

Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	pancreatic cancer
ICD-0-3	C25
Methods	qPCR, Western blot, etc.
Sample	pancreatic cancer tissues, pancreatic cancer cell line Panc-1
Expression Pattern	up-regulated
Function Description	qRT-PCRwas conducted tomeasure the expression of HULC and miR-15a in pancreatic tissues and cells. Cell transfection was used to change the expression of HULC and miR-15a in pancreatic cancer Panc-1 cells. The viability, migration and invasion and apoptosis of Panc-1 cells after relevant transfection were detected using CCK-8 assay, two chamber transwell assay and Guava Nexin assay, respectively. Results found that HULC had a higher expression level, whilemiR-15a had a lower expression level in pancreatic cancer tissues. Overexpression of HULC promoted the proliferation, migration and invasion of Panc-1 cells. Suppression of HULC had opposite effects and dramatically induced cell apoptosis. Moreover, HULC negatively regulated the expression of miR-15a in Panc-1 cells. miR-15a participated in the effects of HULC on Panc-1 cells. Furthermore, overexpression of HULC activated PI3K/AKT pathway in Panc-1 cells by down-regulatingmiR-15a. In conclusion, HULC exerted oncogenic role in pancreatic cancer. Overexpression of HULC promoted the proliferation,migration and invasion of pancreatic cancer cells by down-regulatingmiR-15a and then activating PI3K/AKT pathway.
Pubmed ID	30593805
Year	2018
Title	Long non-coding RNA HULC promotes proliferation, migration and invasion of pancreatic cancer cells by down-regulating microRNA-15a.

External Links