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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	cervical cancer
ICD-0-3	C53
Methods	qPCR, Western blot, Luciferase reporter assay
Sample	Human cervical cancer cell lines HeLa, SiHa and CaSki, cervical cancer tissues and matched adjacent normal cervical tissues
Expression Pattern	up-regulated
Function Description	lncRNA LINC01535 physically binds miR-214, relieves the repressive roles of miR-214 on its target EZH2, and therefore up-regulates EZH2 protein expression. Intriguingly, we also found that EZH2 directly represses the expression of miR-214. Thus, miR-214 and EZH2 form double negative regulatory loop. Through up-regulating EZH2, LINC01535 further represses miR-214 expression. Functional experiments showed that enhanced expression of LINC01535 promotes cervical cancer cell growth, migration and invasion in vitro and cervical cancer xenograft growth in vivo. Reciprocally, LINC01535 knock- down suppresses cervical cancer cell growth, migration and invasion. Activation of the miR-214/EZH2 regulatory loop by overexpression of miR-214 or silencing of EZH2 re- verses the roles of LINC01535 in promoting cervical canc`er cell growth, migration and invasion in vitro and cervical cancer xenograft growth in vivo. Clinically, LINC01535 is significantly up-regulated in cervical cancer tissues and correlated with advanced clini- cal stage and poor prognosis. Moreover, the expression of LINC01535 is reversely as- sociated with the expression of miR-214 and positively associated with the expression of EZH2 in cervical cancer tissues.
Pubmed ID	31273925
Year	2019
Title	Long non-coding RNA LINC01535 promotes cervical cancer progression via targeting the miR-214/EZH2 feedback loop

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