Basic Information
| LncRNA/CircRNA Name | lncRNA-NEF |
| Synonyms | NA |
| Region | NA |
| Ensemble | NA |
| Refseq | NA |
Classification Information
| Regulatory Mechanism | Biological Function | Clinical Application | |||
|---|---|---|---|---|---|
| TF | Immune | Survival | |||
| Enhancer | Apoptosis | Drug | |||
| Variant | Cell Growth | Circulating | |||
| MiRNA | EMT | Metastasis | |||
| Methylation | Coding Ability | Recurrence |
Cancer&Entry Information
| Cancer Name | hepatocellular carcinoma |
| ICD-0-3 | C22.0 |
| Methods | RNA-seq, qPCR, Western blot |
| Sample | liver cell lines (MiHA, SK-HEP-1, PLC/PRF/5, Hep3B and Huh7, HepG2, Bel7404) |
| Expression Pattern | down-regulated |
| Function Description | the lncRNA-NEF was transcriptionally activated by EMT suppressor FOXA2 and frequently downregulated in HCC cell lines as well as clinical specimens. Although enhanced expression of lncRNA-NEF did not affect tumor cell growth, ectopic expression of lncRNA-NEF significantly suppressed EMT program and cell migration. Animal studies validated that lncRNA-NEF alleviated in vivo tumor metastasis and protected mice from tumor-induced mortality. Interestingly, we verified that lncRNA-NEF acted as a novel activator of its neighbor gene FOXA2, which formed a positive feedback loop. Subsequent studies revealed that lncRNA-NEF physically interacted with B-catenin to increase the binding of GSK3B with B-catenin and therefore promoted the inhibitory phosphorylation of B-catenin, leading to the suppression on Wnt/B-catenin signaling and activation of FOXA2 expression. |
| Pubmed ID | 29311643 |
| Year | 2018 |
| Title | LncRNA-NEF antagonized epithelial to mesenchymal transition and cancer metastasis via cis-regulating FOXA2 and inactivating Wnt/B-catenin signaling. |
External Links
| Links for lncRNA-NEF | GenBank HGNC NONCODE |
| Links for hepatocellular carcinoma | OMIM COSMIC |