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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	hepatocellular carcinoma
ICD-0-3	C22.0
Methods	qPCR, Western blot, Luciferase reporter assay, in vitro knockdown, RIP
Sample	hepatocellular carcinoma tissues, cell lines (Hep3B, LM3, MHCC97L, SK-hep1, HepG2, LO2, and HEK-293T)
Expression Pattern	up-regulated
Function Description	NEAT1 was significantly increased in human HCC cell lines. Meanwhile, we observed that hsa-miR-139-5p was greatly decreased in HCC cells, which suggested a negative correlation between NEAT1 and hsa-mir-139-5p. In addition, NEAT1 downregulation can restrain HCC cell growth, migration, and invasion. In addition, LV-shNEAT1 can induce HCC cell apoptosis and inhibit cell migration, invasion. Consistently, overexpression of hsa-mir-139-5p exerted a similar phenomenon. Dual-luciferase reporter assay, RIP assay, and RNA pull-down assay confirmed that NEAT1 can function as a ceRNA by sponging hsa-mir-139-5p. In addition, TGF-?1 was identified as a downstream target of hsa-mir-139-5p and hsa-mir-139-5p overexpression was able to suppress TGF-?1 levels. Furthermore, it was indicated that TGF-?1 inhibition can inhibit HCC cell growth, migration, and invasion ability.
Pubmed ID	29797561
Year	2018
Title	NEAT1 Upregulates TGF-?1 to Induce Hepatocellular Carcinoma Progression by Sponging hsa-mir-139-5p

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