Basic Information
| LncRNA/CircRNA Name | PCAT1 |
| Synonyms | LPCAT1, AGPAT10, AGPAT9, AYTL2, LPCAT-1, PFAAP3, lpcat, lysoPAFAT, PCAT-1 |
| Region | GRCh38_8:126556323-127419050 |
| Ensemble | ENSG00000253438 |
| Refseq | NR_045262 |
Classification Information
| Regulatory Mechanism | Biological Function | Clinical Application | |||
|---|---|---|---|---|---|
| TF | Immune | Survival | |||
| Enhancer | Apoptosis | Drug | |||
| Variant | Cell Growth | Circulating | |||
| MiRNA | EMT | Metastasis | |||
| Methylation | Coding Ability | Recurrence |
Cancer&Entry Information
| Cancer Name | hepatocellular carcinoma |
| ICD-0-3 | C22.0 |
| Methods | qPCR, luciferase reporter assays etc. |
| Sample | cell lines (SMMC7721, Huh7) |
| Expression Pattern | up-regulated |
| Function Description | Here, we report how miRNAs regulate PCAT-1 expression and also investigate the biological significance of this regulation in hepatocellular carcinoma (HCC). We found that miR-215, a P53-inducible miRNA, is a key regulator of PCAT-1 expression in HCC and identified an interaction between miR-215 and PCAT-1 in dual luciferase reporter gene assays. We also found that posttranscriptional silencing of PCAT-1 by miR-215 or PCAT-1 siRNAs significantly inhibited proliferation of HCC cells and, conversely, that inhibition of endogenous miR-215 upregulated PCAT-1 expression and promoted cell viability. The tumor-suppressing role of miR-215 was further confirmed in an in vivo mouse HCC xenograft model. Of note, gene profiling assays suggested that the kinase CRKlike proto-oncogene, adaptor protein (CRKL) is a potential downstream target of the miR-215- PCAT-1 axis in HCC, and we demonstrated that CRKL silencing significantly suppresses cell proliferation. |
| Pubmed ID | 28887306 |
| Year | 2017 |
| Title | The long noncoding RNA PCAT-1 links the microRNA miR-215 to oncogene CRKL-mediated signaling in hepatocellular carcinoma |
External Links
| Links for PCAT1 | GenBank HGNC NONCODE |
| Links for hepatocellular carcinoma | OMIM COSMIC |