Basic Information
| LncRNA/CircRNA Name | SNHG14 |
| Synonyms | NA |
| Region | GRCh38_15:24978583-25419462 |
| Ensemble | ENSG00000224078 |
| Refseq | NR_146177 |
Classification Information
| Regulatory Mechanism | Biological Function | Clinical Application | |||
|---|---|---|---|---|---|
| TF | Immune | Survival | |||
| Enhancer | Apoptosis | apoptosis | Drug | Trastuzumab | |
| Variant | Cell Growth | Circulating | 2 | ||
| MiRNA | EMT | Metastasis | |||
| Methylation | Coding Ability | Recurrence |
Cancer&Entry Information
| Cancer Name | breast cancer |
| ICD-0-3 | C50 |
| Methods | Microarray, qPCR, Western blot, Luciferase reporter assay, in vitro knockdown |
| Sample | breast cancer tissues, serum, cell lines(SKBR-3 and BT474) |
| Expression Pattern | up-regulated |
| Function Description | An lncRNA microarray assay followed by reverse transcription-quantitative polymerase chain reaction analysis identified that lncRNA-small nucleolar RNA host gene 14 (SNHG14) was upregulated in trastuzumab-resistant cells when compared with parental breast cancer cells. Functional experimentation demonstrated that knockdown of lncRNA-SNHG14 potently promoted trastuzumab-induced cytotoxicity. Furthermore, extracellular lncRNA-SNHG14 was able to be incorporated into exosomes and transmitted to sensitive cells, thus disseminating trastuzumab resistance. Treatment of sensitive cells with exosomes highly expressing lncRNA-SNHG14 induced trastuzumab resistance, while knockdown of lncRNA-SNHG14 abrogated this effect. LncRNA-SNHG14 promotes trastuzumab resistance by targeting Bcl-2/Bax signaling, inducing the suppression of apoptotic proteins expression and inhibition of cell apoptosis. Furthermore, the expression level of serum exosomal lncRNA-SNHG14 was upregulated in patients who exhibited resistance to trastuzumab, compared with patients exhibiting a response. Western blot analysis demonstrated an increase in EMT-relevant protein expression in trastuzumab-resistant cells compared with parental cells. Increased expression of exosomal SNHG14 was markedly associated with distant metastasis, lymph node metastasis and cardiac toxicity (P<0.01 for all). |
| Pubmed ID | 30015837 |
| Year | 2018 |
| Title | Exosome-mediated Transfer of lncRNA-SNHG14 Promotes Trastuzumab Chemoresistance in Breast Cancer |
External Links
| Links for SNHG14 | GenBank HGNC NONCODE |
| Links for breast cancer | OMIM COSMIC |