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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug	DDP,Cisplatin
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	non small cell lung cancer
ICD-0-3	C34
Methods	qPCR, Western blot, etc.
Sample	Human NSCLC cell line A549 cells, as well as cisplatinresistant A549/DDP cells
Expression Pattern	up-regulated
Function Description	Results showed that the IC50 of DDP was 16.20 2.27 ?mol/L and 69.72 4.83 ?mol/L in A549 and A549/ DDP cells, respectively. Compared with the A549 group, the expressions of N cadherin, vimentin, and Snail was significantly upregulated in A549/DDP group, but E cadherin was significantly downregulated. Compared with the shCon group, the abundance of N cadherin, vimentin, and Snail was significantly downregulated in short hairpin RNA UCA1 (shUCA1) group, while E cadherin was significantly upregulated. Cell migration and invasion were significantly suppressed and IC50 was reversed to 16.20 2.27 ?mol/L in the shUCA1 group. Silencing lncRNA UCA1 inhibited the migration and invasion of A549/DDP cells and reversed the resistance of A549/DDP cells to DDP. The mechanism might be related to downregulation of epithelial mesenchymal transition, which will provide a new direction for the treatment of non small cell lung cancer with cisplatin.
Pubmed ID	30652341
Year	2019
Title	Silence of??lncRNA UCA1 rescues drug resistance of cisplatin to non-small-cell lung cancer cells

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