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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	bladder cancer
ICD-0-3	C67
Methods	qPCR, Western blot, Luciferase reporter assay, in vitro knockdown
Sample	Human bladder cancer cell lines BIU87, T24 and 79 5637, SV-HUC-1 cells, a SV-40 im- 83 mortalized normal human uroepithelial cell line, cancerous and non-cancerous 60 tissue
Expression Pattern	up-regulated
Function Description	LncRNA-UCA1 is highly expressed in bladder cancer tissues. In cellular experiments, let-7e mimic reduced the luciferase activity in bladder cancer cells treated with the pGL3-basic vector expressing HMGA2 and also inhibited the expression of HMGA2 in bladder cancer cells. Mechanically, delivering plasmid DNA encoding UCA1 (pcDNA-UCA1) was shown to significantly restrain the activity of let-7e, thereby increasing the expression of HMGA2. Knockdown of UCA1 by siRNA or elevated expression of let-7e decreased the proliferative, migratory and invasive potential in bladder cancer cells, along with promoted apoptosis. Importantly, let-7e mimic was found to induce the apoptosis in bladder cancer cells after UCA1 knockdown. For in vivo analysis, we observed that let-7e mimic could decrease the growth of tumor xenografts in mice with subcutaneous injections of siRNA against UCA1
Pubmed ID	31306103
Year	2019
Title	Long non-coding RNA UCA1 regulates tumor growth by impairing let-7e-dependent HMGA2 repression in bladder cancer

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