Search Result

Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	bladder cancer
ICD-0-3	C67
Methods	qPCR, Western blot, ChIP, Luciferase reporter assay etc.
Sample	cell lines (BLZ-211 and BLS-211)
Expression Pattern	differential expression
Function Description	To verify if Ets-2 induced UCA1 gene expression was involved in apoptosis we studied another bladder TCC cell line, called BLS-211. BLS-211 is lack of endogenous UCA1 expression and is derived from the same patient as BLZ-211 cell line. Interestingly, after knocking down the Ets-2 gene, we didn?? observed subsequent apoptosis in BLS-211 cells. Additionally, we counted the apoptotic cells using flow cytometry in both cell lines separately. Consistent with the fluorescence microscope results, the flow cytometry results revealed a significant increase of cell pro-apoptosis (upper right quadrant) after Ets-2 knockdown in BLZ-211 cells while no increase of pro-apoptosis was observed after Ets-2 knockdown in BLS-211 cells. These results suggested that UCA1 may be involved in the cell apoptosis induced by the suppression of the Ets-2.
Pubmed ID	24069250
Year	2013
Title	Ets-2 regulates cell apoptosis via the Akt pathway, through the regulation of urothelial cancer associated 1, a long non-coding RNA, in bladder cancer cells.

External Links