Basic Information
| LncRNA/CircRNA Name | YWHAE |
| Synonyms | NA |
| Region | NA |
| Ensemble | NA |
| Refseq | NA |
Classification Information
| Regulatory Mechanism | Biological Function | Clinical Application | |||
|---|---|---|---|---|---|
| TF | Immune | Survival | |||
| Enhancer | Apoptosis | Drug | |||
| Variant | Cell Growth | Circulating | |||
| MiRNA | EMT | Metastasis | |||
| Methylation | Coding Ability | Recurrence |
Cancer&Entry Information
| Cancer Name | colorectal cancer |
| ICD-0-3 | C19.9 |
| Methods | qPCR, Western blot, Luciferase reporter assay |
| Sample | HT29 and HCT116 cell lines, SW480 and HEK293-T cells , colorectal normal, colorectal cancer tissues |
| Expression Pattern | up-regulated |
| Function Description | YWHAE gene is upregulated in colorectal cancer specimens. Additionally, bioinformatics analysis suggested that YWHAE-lncRNA sponges miR-323a-3p and miR-532-5p that were predicted to target K-Ras 3`UTR sequence. Overexpression of YWHAElncRNA, resulted in upregulation of K-Ras gene expression while, overexpression of both miR-323a-3p and miR-532-5p had an inverse effect, detected by RT-qPCR. Consistently, western blot analysis confirmed that YWHAE-lncRNA overexpression upregulated K-Ras/Erk1/2 and PI3K/Akt signaling pathways while, miR-323a-3p and miR-532-5p overexpression suppressed both pathways in HCT116 cells. Furthermore, dual luciferase assay validated the direct interaction of miR-323a-3p and miR-532-5p with K-Ras 3`UTR sequence, and supported the sponging effect of YWHAE-lncRNA over both miRNAs. |
| Pubmed ID | 31238337 |
| Year | 2019 |
| Title | YWHAE long non-coding RNA competes with miR-323a-3p and miR-532-5p through activating K-Ras/Erk1/2 and PI3K/Akt signaling pathways in HCT116 cells |
External Links
| Links for YWHAE | GenBank HGNC NONCODE |
| Links for colorectal cancer | OMIM COSMIC |