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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	gastric cancer
ICD-0-3	C16
Methods	qRT-PCR , Western blotting , cell apoptosis assay , Luciferase activity assay , RNA pull-down assay , cell invasion assay
Sample	gastric cancer cell lines( AGS, BGC-823, HGC-27, MGC-803, MKN-45 and SGC-7901), and human gastric epithelial cell line GES-1
Expression Pattern	down-regulated
Function Description	This study showed that circ_SPECC1 was down-regulated in various GC cell lines, significantly inhibited GC cell proliferation and invasion, and promote apoptosis, which might play an anti-oncogene role.Circ_SPECC1 was mainly located in the cytoplasm, and its sequence contained multiple potential binding sites of miR-526b.miR-526b could bind directly to circ_SPECC1. miR-526b was also down-regulated in GC cells, and one of its important target genes was KDM4A. Both circ_SPECC1 and miR-526b inhibited the expression of KDM4A and its downstream effector YAP1, but miR-526b inhibitors terminated the above-mentioned inhibition of circ_SPECC1, and KDM4A overexpression reversed the inhibition of circ_SPECC1 and miR-526b on YAP1 expression. Both miR-526b and KDM4A siRNA inhibited GC cell proliferation and invasion, and promote apoptosis; KDM4A overexpression had the opposite effects, and significantly blocked the regulation of miR-526b on cell growth and invasion.
Pubmed ID	31349968
Year	2019
Title	Circ_SPECC1 Enhances the Inhibition of miR-526b on Downstream KDM4A/YAP1 Pathway to Regulate the Growth and Invasion of Gastric Cancer Cells

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