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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	gastric cancer
ICD-0-3	C16
Methods	qRT-PCR , luciferase reporter assay , western blot , CCK?? assay , colony formation assay , hoechst staining , transwell assay , Immunohistochemistry
Sample	GC tissues and adjacent non??umour tissues ,GC cell lines (KN??5, MGC??03, AGS and SGC??901) and human gastric epithelial cell line (GES????)?
Expression Pattern	up-regulated
Function Description	Patients with a high hsa_circ_006100 level or low miR-195 level had tumours with a high TNM stage, poor cellular differentiation and lymph node metastasis. miR-195 was targeted and inhibited by hsa_circ_006100. Overexpression of hsa_circ_006100 enhanced cellular viability and proliferation, while miR-195 suppressed hsa_circ_006100-enhanced cell growth and induced apoptosis in MGC-803 and AGS cells.Forced hsa_circ_006100 expression promoted the migration and invasion of MGC-803 and AGS cells, while those activities were inhibited by miR-195. Mechanistically, GPRC5A was predicted as a target of miR-195 and was upregulated in gastric cancer.In vivo studies showed that knockdown of hsa_circ_006100 delayed tumour growth, reduced PCNA expression and upregulated miR-195 and BCL-2 expression which was restored by miR-195 inhibition due to GPRC5A/EGFR signalling, and changed the EMT phenotype in vivo.
Pubmed ID	31318114
Year	2019
Title	Elevated Levels of hsa_circ_006100 in Gastric Cancer Promote Cell Growth and Metastasis via miR-195/GPRC5A Signalling

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