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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	NA	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	liver cancer
ICD-0-3	C22.0
Methods	qRT-PCR , western blot , luciferase reporter assay , RNA-seq , northern blotting , RNase-R treatment, sanger sequencing , colony formation assay , flow cytometry analysis , wound healing assay , cell invasion assay , co-immunoprecipitation , mass spectrometry
Sample	HCC tissues and corresponding adjacent normal tissues ,cell lines(SW620, Huh7, HepG2, and PLC/PRF/5 )
Expression Pattern	up-regulated
Function Description	Circ?-catenin is predominantly localized in the cytoplasm and displays resistance to RNase-R treatment. We find that circ?-catenin is highly expressed in liver cancer tissues when compared to adjacent normal tissues. Silencing of circ?-catenin significantly suppresses malignant phenotypes in vitro and in vivo, and knockdown of this circRNA reduces the protein level of ?-catenin without affecting its mRNA level. We show that circ?-catenin affects a wide spectrum of Wnt pathway-related genes, and furthermore, circ?-catenin produces a novel 370-amino acid ?-catenin isoform that uses the start codon as the linear ?-catenin mRNA transcript and translation is terminated at a new stop codon created by circularization. We find that this novel isoform can stabilize full-length ?-catenin by antagonizing GSK3?-induced ?-catenin phosphorylation and degradation, leading to activation of the Wnt pathway.
Pubmed ID	31027518
Year	2019
Title	Translation of the Circular RNA Circ?-Catenin Promotes Liver Cancer Cell Growth Through Activation of the Wnt Pathway

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