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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	Colon Cancer
ICD-0-3	C18
Methods	qRT-PCR, Western blotting etc.
Sample	Sixty-seven colon cancer tissue samples, Human colonic neoplasm cell lines HCT 116, SW1417, HT-29, and KM12 and the human normal colonic epithelial cell line NCM460
Expression Pattern	down-regulated
Function Description	CCAT1 in colon cancer tissues was higher than that in noncancer tissues, and the levels of CCAT1 in HCT 116, SW1417, HT-29, and KM12 cell lines were higher than those in the human normal colon epithelial NCM460 cell line. Moreover, the expression levels of CCAT1 were high in HCT 116/5-FU and HT-29/5-FU cell lines, whose apoptosis rates induced by 5-FU were lower than those in corresponding parental cells. The results of qRT-PCR and CCK-8 assay showed that enhancement of lncRNA CCAT1 expression levels in HCT 116 and HT-29 cell lines increased their IC50?of 5-FU and decreased their apoptosis rates. Meanwhile, siRNA-CCAT1 effectively inhibited the expression of CCAT1 and enhanced the 5-FU-sensitivity of HCT 116/5-FU and HT-29/5-FU, in which apoptosis rates were increased at the same time.
Pubmed ID	31039730
Year	2019
Title	Downregulation of lncRNA CCAT1 Enhances 5-fluorouracil Sensitivity in Human Colon Cancer Cells

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