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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	breast cancer
ICD-0-3	C50
Methods	qRT-PCR, Dual luciferase assay, Cell proliferation assay, EdU incorporation assay, Western blotting, Transwell assays, Wound-healing assays, Flow cytometric analysis
Sample	breast cancer tissues, breast cancer cell lines (BT-549, ZR-75-30, MDA-MB-231, MCF-7, BT-474, Hs578T, T-47D, BT-549 and BT-474)
Expression Pattern	up-regulated
Function Description	circ_0006528 expression was significantly upregulated in human breast cancer tissues compared with that in adjacent non-tumourous tissues and was significantly associated with advanced tumour-node-metastasis (TNM) stage and poor prognosis. In addition, we found that in breast cancer cells, circ_0006528 could promote DNA synthesis and cell proliferation, invasion and migration. Downregulating circ_0006528 induced G2 phase arrest and cell apoptosis. Further mechanistic studies revealed that circ_0006528 could sponge endogenous miR-7-5p and inhibit its activity. We also identified Raf1, which activates the MAPK/ERK signalling pathway, as a target of miR-7-5p and determined that circ_0006528 promotes breast cancer growth, invasion and migration by promoting the expression of Raf1 and activates the MAPK/ERK pathway.
Pubmed ID	30520151
Year	2018
Title	hsa_circRNA_0006528 as a competing endogenous RNA promotes human breast cancer progression by sponging miR-7-5p and activating the MAPK/ERK signalling pathway?

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