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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis	apoptosis	Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	glioma
ICD-0-3	NA
Methods	qRT??CR, Western blot, Cell cycle measured with propidium iodide by flow cytometry, Colony formation assay, Temozolomide toxicity assay, Annexin V??PC??/PI apoptosis assay, Scratch test, Transwell invasion test, Animal studies
Sample	glioblastoma tissues, cell lines U251, U87, and HEB cells
Expression Pattern	up-regulated
Function Description	the expression of miR-181a was significantly decreased in glioma tissues and cell lines compared with normal brain tissues and normal gliocyte, respectively, and the expression of hsa_circ_0076248 and SIRT1 demonstrated the opposite. Bioinformatics analysis identified hsa_circ_0076248 could sponge miR-181a, and miR-181a could target the mRNA of SIRT1. Our results verified that downregulating hsa_circ_0076248 or upregulating miR-181a could depress the proliferation and invasion of glioma in vitro and in vivo. The experiment also showed that downregulating hsa_circ_0076248 or upregulating miR 181a could remarkably promote the temozolomide chemotherapy sensitivity. Furthermore, Western blot analysis testified that downregulating hsa_circ_0076248 or upregulating miR 181a could promote the expression of p53 and SIRT1. In summary, our study sheds light on the regulatory mechanism of hsa_circ_0076248 in glioma growth and invasion via sponging miR 181a, which downregulates the SIRT1 expression and also suggests that hsa_circ_0076248, miR-181a, and SIRT1 may serve as potential therapeutic targets for glioma.
Pubmed ID	30506951
Year	2018
Title	Circular RNA hsa_circ_0076248 promotes oncogenesis of glioma by sponging miR??81a tomodulate SIRT1 expression

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