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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	lung adenocarcinoma
ICD-0-3	C34
Methods	qRT-PCR, Cell proliferation assays, Transwell migration and invasion assays, Animal experiments, Western blot, Immunofluorescence assay, FISH, circRNA in vivo precipitation (circRIP), Luciferase reporter assay
Sample	LUAD tissues, LUAD cell lines (H1299, A549, SPC-A1, and Calu3) and human normal lung epithelial cell line BEAS-2B
Expression Pattern	down-regulated
Function Description	Firstly, the downregulation of circ_0006427 in LUAD tissues and cell lines was revealed by microarray analysis and qRT-PCR analysis. And we also confirmed the circ_0006427 as a prognostic target in LUAD patients. Functionally, overexpression of circ_0006427 effectively suppressed cell proliferation, migration and invasion. Mechanistically, circ_0006427 was found to be predominantly located in the cytoplasm of LUCA cell, and was further revealed to positively regulate DKK1 in LUAD by sponging miR-6783e3p. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis and western blot analysis revealed that circ_0006427 inactivated Wnt/b-catenin signaling pathway by upregulating DKK1. At last, rescue assays proved the function of circ_0006427/miR-6783e3p/DKK1 axis in LUAD progression.
Pubmed ID	30470570
Year	2018
Title	CircRNA has_circ_0006427 suppresses the progression of lung adenocarcinoma by regulating miR-6783e3p/DKK1 axis and inactivating Wnt/b-catenin signaling pathway

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