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Basic Information

Classification Information

Regulatory Mechanism		Biological Function		Clinical Application
TF		Immune		Survival
Enhancer		Apoptosis		Drug
Variant		Cell Growth		Circulating
MiRNA		EMT		Metastasis
Methylation		Coding Ability		Recurrence

Cancer&Entry Information

Cancer Name	hepatocellular cancer
ICD-0-3	NA
Methods	Mouse experiments, Western blotting, qPCR, Dual luciferase analysis, ChIP, RNA-IP assay, Cell viability and colony formation assay, ELISA
Sample	liver cancer tissues, liver cancer cell lines Bel-7402, SMMC-7721, Huh7, HepG2, Hep1, Bel-7404, the hepatocyte lines THLE-3, HL-7702, Serum samples
Expression Pattern	up-regulated
Function Description	circRNA_104075 (circ_104075) was highly expressed in HCC tissues, cell lines and serum. Mechanistically, HNF4a bound to the 1409 to 1401 region of the circ_104075 promoter to stimulate the expression of circ_104075. Moreover, circ_104075 acted as a ceRNA to upregulate YAP expression by absorbing miR-582-3p. Interestingly, an N6 - methyladenosine (m6 A) motif was identified in the 353 357 region of YAP 3 UTR, and this m6 A modification was essential for the interaction between miR-582-3p and YAP 3 UTR. Further, the diagnostic performance of circ_104075 was evaluated. The area under the receiver operating characteristic (AUC-ROC) for circ_104075 was 0.973 with a sensitivity of 96.0% and a specificity of 98.3%. Collectively, we determined that circ_104075 was highly expressed in HCC and elucidated its upstream and downstream regulatory mechanisms.
Pubmed ID	30361504
Year	2018
Title	circRNA_104075 stimulates YAPdependent tumorigenesis through the regulation of HNF4a and may serve as a diagnostic marker in hepatocellular carcinoma

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