Thus, we concluded that FOXF1-AS1 may promote migration and invasion of OS cells through the FOXF1/MMP-2/-9 pathway. Taken together, these findings demonstrated the underlying mechanism of FOXF1-AS1 in the regulation of OS progression and provide a novel potential target in the OS therapy.OS patients with lower expression of FOXF1 had longer overall survival time than those with higher expression. *P < 0.05, **P < 0.01.LncRNA FOXF1-AS1 is one of antisense long noncoding RNAs and its conjugate gene FOXF1 (forkhead transcription factor 1) belongs to the 43 members of transcription factor FOX gene family 31.we speculated the cytoplasmic lncRNA FOXF1-AS1 may regulate the expression of FOXF1 and MMPs at the posttranscriptional level as an endogenous competing RNA to sponge the related miRNAs.