FOXD2- AS1 expression was significantly increased in CRC tissues as well as in CRC cell lines. down-regulation of FOXD2-AS1 suppressed cell, proliferation, invasion and migration in vitro. FOXD2-AS1 promoted the progression of CRC by regulating EMT and Notch signaling pathway. Thus, targeting FOXD2-AS1 may be an effective strategy for CRC treatment.Although recent advances have been made in diagnostic procedures and therapeutic strategies, the 5-year survival rate for metastatic CRC is only 10-15%3,4.Waltham,MA, USA) Dulbeccoâ??s modified Eagleâ??s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), Sigma-Aldrich.