Finally, the in vivo experiment revealed that tumor growth and metastasis could also be inhibited by HOTAIR shRNA. Our present study elucidated the regulating role of HOTAIR/miR-326 axis in cervical cancer progression and provided a new potential therapeutic strategy for cervical cancer. Although many therapeutic means such as surgery, chemotherapy, radiotherapy have been applied to cervical treatment, the prognosis and the five-year survival rate are still not satisfactory4. Previous researches had shown that HOTAIR took part in forming a complex with Polycomb repressive complex 2 (PRC2) which finally result in histone H3 lysine methylation, cancer invasion and metastasis29-31.