The silencing of HOTAIR in liver cancer cells induced the inhibition of cell proliferation and promotion of apoptosis.Several pathways such as extracellular matrix receptor interaction, focal adhesion, pathways in cancer were annotated with the HOTAIR and coexpression genes. HOTAIR might be an oncogene in HCC. It functions though promoting tumor cell growth and inhibiting apoptosis. HOTAIR may potentially be involved in HCC metastatic progression by several pathways correlated to cell adhesion.higher HOTAIR expression could significantly predict worse overall survival.