Detail
| LncRNA Name | NCK1-AS1 |
| Synonyms | NA |
| Region | GRCh38_3:136841726-136862054 Sequence |
| Ensembl | ENSG00000239213 |
| RefSeq | NA |
| Circulating | ✘ |
| Drug-resisitant | ✘ |
| Prognostic | ✔ |
| MiRNA | ✔ |
| Variant | ✘ |
| TF | ✔ |
| Methylation | ✘ |
| Cancer Name | cervical cancer |
| ICD-0-3 | C53 |
| Methods | Microarray, qRT-PCR, Western blot, Luciferase reporter assay, in vitro knockdown |
| Sample | cervical squamous cell carcinoma tissues, cervical cancer cell lines (HeLa, C33A and SiHa and CaSki) |
| Expression Pattern | up-regulated |
| Function Description | Functionally,we screened the CC-associated lncRNA NCK1-AS1 as a new candidate lncRNA and regulator which promotes development and progression in CC. qRT-PCR and RNA in situ hybridization (RISH) results showed that NCK1-AS1 was significantly up-regulated in 77.4% of the CC tissue group compared with the normal group.Interestingly,we demonstrated that transcription factor SP1 directly binds to the promoter to activate NCK1-AS1 expression in SiHa cells.In vitro and in vivo assays of silencing NCK1-AS1 significantly inhibited cell proliferation and invasion, with induction of cell arrest in S phase of the cell cycle. NCK1-AS1 functioned as a molecular sponge for miR-6857, antagonizing its ability to repress CDK1/6 protein translation. |
| Pubmed ID | 29416014 |
| Year | 2018 |
| Title | LncRNA NCK1-AS1 promotes proliferation and induces cell cycle progression by crosstalk NCK1-AS1/miR-6857/CDK1 pathway. |
| External Links |
| Links for NCK1-AS1 | GenBank HGNC lncrnadb Noncode |
| Links for cervical cancer | Omim Cosmic |
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