In the present study, we revealed that prostrate androgen-regulated transcript1 (PART-1) was highly expressed in colorectal cancer cells and tissues , and knockdown of PART-1 suppressed cell proliferation and metastasis, both in vitro and in vivo. In addition, PART-1 functioned as a ceRNA of DNMT3A,by sponging miR-143. Finally, PART-1 induced tumor progression by regulating DNMT3A. The Kaplan-Meier log-rank test was used to compare survival curves.Several studies have reported that miR-143 could regulate tumor progression through direct interaction with DNMT3A. As an enzyme modulating DNA methylation, DNMT3A can exert diverse epigenetic regulations.