up-regulated PlncRNA-1 in CRC tissues and cells promoted cell proliferation by accelerating cell cycle process and inhibiting cell apoptosis in vitro, enhanced tumor growth and matastasis in vivo and was associated with cell migration and invasion, EMT process of CRC cells. In addition, PlncRNA-1 was a target of miR-204 and enhanced the expression of an endogenous miR-204 target, MMP9 in CRC cells.Furthermore, we found that PlncRNA-1 activates Wnt/B-catenin pathway through the miR-204 in CRC cells.the PlncRNA-1/miR-204/ Wnt/B-catenin regulatory network may shed light on tumorigenesis in CRC. PlncRNA-1 may act as a ceRNA to regulate the expression level of MMP9 in a miR-204 dependent manner.