we firstly identified that PlncRNA-1 expression levels were prominently augmented in glioma patient tissues and glioma cell lines compared with adjacent noncancerous tissue and normal cells, respectively. Moreover, Kaplan-Meier survival analysis indicated that glioma patients with high PlncRNA-1 expression had shorter overall survival (OS) and progression-free survival (PFS) than those with low PlncRNA-1 expression. Furthermore, loss-of-function assay showed that PlncRNA-1 knockdown dramatically reduced cell proliferation, colony formation, and promoted apoptosis of glioma cell lines. In addition, overexpression of PlncRNA-1 promoted cell proliferation, stimulated cell colony formation, and inhibited cell apoptosis in NHA cells. Mechanically, our results showed that PlncRNA-1 significantly promoted activation of the Notch signal pathway through regulation of Notch-1, Jag-1, and Hes-1 expression.