XIST expression level was upregulated in colon cancer tissues and cell lines.In addition,the growth rate of cells transfected with si-XIST was significantly decreased compared to that with si-NC, which was reversed by miR-34a targeted with 3'-UTR. Moreover, miR-34a suppressed the expression of WNT1 by binding with the 3'-UTR, which interact with WNT1 to inhibit the proliferation of cells.Furthermore, miR-34a inhibitor rescued the dysregulation of WNT1, B-catenin,cyclinD1,c-Myc and MMP-7 by si-XIST.Besides, XIST knockdown inhibited tumor growth in vivo. In short, the current study suggests XIST plays as an important role in colon cancer progression targeted by miR-34a via Wnt/B-catenin signaling pathway,providing a novel insight for the pathogenesis and underlying therapeutic target for colon cancer.