ZFAS1 expression was significantly upregulated in bladder cancer tissues and cell lines.Furthermore, Kaplan-Meier analysis revealed that high ZFAS1 expression was significantly associated with unfavorable progression free survival (PFS) and overall survival(OS) of bladder cancer patients. Moreover, silencing of ZFAS1 expression could markedly suppress bladder cancer cells proliferation and colony formation, arrest cell cycle, promote cell apoptosis and inhibit cell migration in vitro. In addition, bioinformatics analysis, luciferase reporter assay,and pull down assay revealed that ZFAS1 straightly interacted with miR-329.Lastly, rescue experiments confirmed that miR-329 inhibitor reversed the tumor suppressing roles of ZFAS1 knockdown on bladder cancer cells.Collectively, our results illuminated that ZFAS1 could serve as an oncogene in the tumorigenesis of bladder cancer,and discovered the functional regulatory network of ZFAS1 sponging miR-329.