a functional role of ZFAS1 on OS growth and metastasis. The expression of ZFAS1 was signifiantly overex- pressed in OS samples and cell lines, and upregulation of ZFAS1 is signifiantly associated with unfavorable prognosis of OS patients. Functional assays also demonstrated that ZFAS1 enhanced the growth and metastatic ability of OS cells in vitro and in vivo.Mechanistically, we found that ZFAS1 positively regulated malignant phenotypes by competitively binding the miR-200b and miR-200c and upregulating BMI1.ZFAS1 also interacted with ZEB2 and regulated ZEB2 protein stability. Furthermore, we demonstrated that SP1 functions as an upstream activated factor of ZFAS1.ZFAS1 may be a potential therapeutic target for OS tumorigenesis and progression.