DLEU1 was observably intensified in GC tissues and cell lines. And highly expressed DLEU1 was relevant to tumor size, advanced stage of pathology and lymph node metastasis in GC patients. Silenced DLEU1 obviously suppressed proliferation via leading to the cell cycle arrest and inducing cell apoptosis of GC.DLEU1 could recruit LSD1(lysine specific demethylase 1) to the promoter regions of KLF2 and then suppressed its transcription. the oncogenic function mediated by DLEU1 in GC was partly by regulating KLF2.highly expressed DLEU1 means a poorer survival ability for GC patients.