Detail

Chr	NA
start	NA
end	NA
lncRNA name	ANAC
entrez id	NA
hgnc id	NA
ensg id	NA
refseq id	NA
methods	qPCR, Western blot, Luciferase reporter assay etc.
regulated	down-regulated
function description	We find that lncRNA ANCR participates in TGF-B1-induced EMT. By our ChIP and Real-time PCR assays, we reveal that TGF-B1 down-regulates ANCR expression by increasing HDAC3 enrichment at ANCR promoter region, which decreases both H3 and H4 acetylation of ANCR promoter. In addition, by western blot and transwell assays, we indicate that ectopic expression of ANCR partly attenuates the TGF-B1-induced EMT. Downstream, ANCR inhibits breast cancer cell migration and breast cancer metastasis by decreasing RUNX2 expression in vitro and in vivo. Thus, our study identifies ANCR, as a new TGF-B downstream molecular, is essential for TGF-B1-induced EMT by decreasing RUNX2 expression. These results implicate that ANCR might become a prognostic biomarker and an anti-metastasis therapy target for breast cancer. These results demonstrate that ANCR is down-regulated during the TGF-B1-induced EMT in MCF10A cells.
pubmed id	28978036
year	2017
title	LncRNA ANCR down-regulation promotes TGF-B-induced EMT and metastasis in breast cancer.
drug	X
circulating	X
survival	X