Detail

Chr	chr20
start	49278178
end	49295738
lncRNA name	ZFAS1
entrez id	441951
hgnc id	HGNC:33101
ensg id	ENSG00000177410
refseq id	NR_003604
methods	qPCR etc.
regulated	down-regulated
function description	Using human breast cancer cell lines, ZFAS1 was found to be expressed in all cell lines tested, albeit at different levels of abundance. Following subcellular fractionation, human ZFAS1 was found in both nucleus and cytoplasm (as is the mouse orthologue) in an isoform-independent manner. Sucrose gradients based on velocity sedimentation were utilised to separate the different components of total cell lysate, and surprisingly ZFAS1 was primarily co-localised with light polysomes. Further investigation into ribosome association through subunit dissociation studies showed that ZFAS1 was predominantly associated with the 40S small ribosomal subunit. The expression levels of ZFAS1 and of mRNAs encoding several ribosomal proteins that have roles in ribosome assembly, production and maturation were tightly correlated. ZFAS1 knockdown significantly reduced RPS6 phosphorylation.
pubmed id	27871336
year	2016
title	ZFAS1: a long noncoding RNA associated with ribosomes in breast cancer cells.
drug	X
circulating	X
survival	X