Detail

Chr	NA
start	NA
end	NA
strand	NA
Geng Symbol	NA
circRNA name	circFBXW7
methods	qRT-PCR,Luciferase Assay,RIP,Western Blot,etc.
regulated	Down?
Function Description	As shown inFigure 6A, the linearized FBXW7-185aa open readingframe (ORF) and a FLAG tag were cloned into a plasmid (FBXW7-185aa-FLAG). Overexpression of FBXW7-185aa did not affect theexpression level of the circFBXW7 transcript, as measured by qRT-PCR (Figure 6B). Subsequently, we conducted CCK-8, colony forma-tion, and transwell assays to assess the influence of FBXW7-185aa onTNBC cell growth and proliferation. FBXW7-185aa significantly in-hibited the growth, colony-forming, and migration abilities ofBT549 cells (Figures 6C�C6E). Additionally, the prooncogenic effectenhanced by sh-circFBXW7 was reversed after cotransfection withmiR-197-3p inhibitors and the FBXW7-185aa protein expressionplasmid (Figures 6F and 6G). Western blot analysis revealed thatoverexpression of FBXW7-185aa increased the abundance ofFBXW7 and induced c-Myc degradation. Overexpressing USP28reduced the expression of FBXW7 and suppressed FBXW7-185aa-induced c-Myc destabilization (Figure 6H). In summary, circFBXW7sponges miR-197-3p and encodes the FBXW7-185aa protein to sup-press TNBC progression though upregulating FBXW7 expression(Figure 6I).
PMID	31536884
year	2019
Title	circFBXW7 Inhibits Malignant Progression by Sponging miR-197-3p and Encoding a 185-aa Protein in Triple-Negative Breast Cancer.
drug	X
circulating	X
survival	V