Detail

Chr	chr12
start	66597490
end	66622150
strand	+
Geng Symbol	IRAK3
circRNA name	hsa_circ_0005505
methods	Microarray,qRT-PCR,FISH,Luciferase assay,Western blot,RNAi,etc.
regulated	up
Function Description	Results: Microarray analysis and qRT-PCR verified a circRNA termed circGFRA1 that was upregulated in TNBC. Kaplan-Meier survival analysis showed that upregulated circGFRA1 was correlated with poorer survival. Knockdown of circGFRA1 inhibited proliferation and promoted apoptosis in TNBC. Via luciferase reporter assays, circGFRA1 and GFRA1 was observed to directly bind to miR-34a. Subsequent experiments showed that circGFRA1 and GFRA1 regulated the expression of each other by sponging miR-34a.Conclusions: Taken together, we conclude that circGFRA1 may function as a competing endogenous RNA (ceRNA) to regulate GFRA1 expression through sponging miR-34a to exert regulatory functions in TNBC. circGFRA1 may be a diagnostic biomarker and potential target for TNBC therapy.
PMID	29803789
year	2018
Title	CircIRAK3 sponges miR-3607 to facilitate breast cancer metastasis
drug	X
circulating	V
survival	V