Detail

Chr	chr7
start	129774739
end	129774761
ID	MIMAT0000095
miRNA_name	hsa-miR-96-5p
methods	qRT-PCR,Western blot,Luciferase assay,etc.
regulated	differential expression
Function Description	Results: Luciferase reporter assays showed miRNA-96-5p directly targeted FOXO3. Abrogation of miRNA-96-5p by transfection with its inhibitors in breast cancer cells significantly suppressed miRNA-96-5p expression and breast cancer cells proliferation. Western blot revealed that overexpression of miRNA-96-5p substantially reduced FOXO3 protein expression. We used the GEPIA, UALCAN and KM-plotter databases to investigate the expression of FOXO3 in human breast cancer and adjacent normal tissues, and its correlation with survival. In addition, we found that FOXO3 spoiled miR-96-5p induced breast cancer cell proliferation block effecting.Conclusions: miRNA-96-5p may exert a tumor promotion role through negatively regulating tumor suppressor gene FOXO3 and promoting cell proliferation.
PMID	32100957
year	2020
Title	MiRNA-96-5p impacts the progression of breast cancer through targeting FOXO3.
drug	X
circulating	X
survival	V