Detail

Chr	chr17
start	59841266
end	59841337
ID	MI0000077
miRNA_name	hsa-miR-21
methods	qRT-PCR,etc
regulated	up
Function Description	In summary, we successfully developed a single-molecule method for the quantification of circulating miRNA biomarkers. Taking the advantage of the sensitivity of TIRFM-based single-molecule platform and the amplification of CHA, rapid and sensitive miRNA detection with low background signal was readily achieved. With smCHA, synthetic miRNA targets can be detected in solution with an excellent detection limit below 0.02 fM, and the circulating miRNAs can be quantified showing a good agreement with the RT-qPCR as gold standard. Differential levels of circulating miRNAs were discovered between cancer samples and healthy controls. Our method avoids the steps of RNA isolation, PCR amplification, and elaborated result analysis which typically take roughly 3-4 h. smCHA can deliver result of single sample within 0.5 h (10 min for pretreatment, 15 min for CHA, and 5 min for single-molecule counting). Multiplexing can achieved by using different fluorophores or other fingerprinting labels. smCHA would find more applications in biomarker detection and DNA-based biosensing.
PMID	30428976
year	2018
Title	Single-molecule Catalytic Hairpin Assembly for Rapid and Direct Quantification of Circulating miRNA Biomarkers
drug	X
circulating	V
survival	X